Online Stock Market Trading Mahidol University Annual Research Abstracts 2000 41 DICHLOROPHENOL-INDOPHENOL (DCIP) PRECIPITATION TEST FOR DETECTION OF HbE AND HbH : FROM DARK TO DAWN Wanida Ittarat, Kritsana Pathepchotiwong and Apapan Srisarin Department of Clinical Mecorscopy, Faculty of Medical Technology, Mahidol Univesity, Bangkok 10700. Thailand.
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Stock Market Game Key words : hemoglobin, unstable hemoglobin, hemoglobin E, hemoglobin H, Dichlorophenol-indophenol (DCIP) precipitation test, ascorbic acid, vitamin C
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Journal Prime Rate Street Wall Address correspondence to : Assoc Prof. Wanida Ittarat, Ph.d. Department of Clinical Microscopy, Faculty of Medicine Technology, Mahidol University. 2 Pran nok road, Bangkok noi, Bangkok 10700, Thailand. Phone : 66-2-419-7169, Fax : 66-2-412-4110; e-mail : mtwit@mahidol.ac.th.
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Stock Investing Basics Dichlorophenol-indophenol (DCIP) precipitation test is a very simple and useful test for detection of unstable hemoglobin such as hemoglobin E (Hb E) and hemoglobin H (Hb H). DCIP is a synthetic dye which has deep blue color in water. Interpretation of precipitation or cloudy appearance in such dark blue solution is very frustrating especially for those who do not have experiences. The present study is the first report to modify the test by adding 40 M ascorbic acid to clear the deep blue color. Quantitation of the hemoglobin could be done by measuring the absorbance at 800 nm. It was found that the absorbance of homozygous Hb E, heterozygous Hb E and Hb A2A were 1.01 + 0.35, 0.39 + 0.09 and 0.1 + 0.05, respectively . The absorbance was consistent with the level of Hb E determined by hemoglobin electrophoresis and high performance liquid chromatography (HPLC).
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